Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1579, Issue -, Pages 115-120Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2018.10.024
Keywords
Surface enhanced Raman scattering; Thin layer chromatography; Aflatoxins; High sensitivity; Gold colloids
Funding
- National Natural Science Foundations of China [21505057, 61575087, 21605062]
- Natural Science Foundation of Jiangsu Province [BK20150227, BK20151164]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Brand Major of Universities in Jiangsu Province
- Top-notch Academic Programs Project of Jiangsu Higher Education Institution (TAPP)
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A fast and facile method was developed for on-site detection of aflatoxins (AFs) in moldy agricultural products using thin layer chromatography combined with surface-enhanced Raman spectroscopy (TLC-SERS). Four different AFs were successfully separated by TLC and then a small portable Raman spectrometer, with gold colloids as the SERS-active substrate, was applied to identify the separated spots. TLC-SERS application to on-site detection of AFs was systematically investigated. Qualitative and quantitative AF detection was found to be easily accomplished and limits of detection were estimated to be 1.5 x 10(-6), 1.1 x 10(-5), 1.2 x 10(-6), and 6.0 x 10(-7) M for AFE(1), AFB(2), AFG(1), and AFG(2), respectively. The proposed method was also highly selective, enabling successful AF identifications in complex extracts from moldy peanuts. The study showed that TLC-SERS could be effectively used for separation and detection of these four AFs, demonstrating good prospects for on-site qualitative screening of agricultural products. (C) 2018 Elsevier B.V. All rights reserved.
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