Journal
JOURNAL OF CELLULAR PHYSIOLOGY
Volume 234, Issue 5, Pages 6679-6687Publisher
WILEY
DOI: 10.1002/jcp.27410
Keywords
growth plate (GP) cartilage; Indian hedgehog (Ihh); intervertebral disc (IVD); notochordal cells; runt-related transcription factor 2 (Runx2)
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Funding
- National Institute of Arthritis and Musculoskeletal and Skin Diseases [R01AR070222, R01AR054465]
- National Natural Science Foundation of China [81700997]
- Shenzhen Science and Technology Innovation Committee, China [JCYJ20160331114205502]
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Runx2 plays an essential role in embryonic disc tissue development in mice. However, the role of runt-related transcription factor 2 (Runx2) in postnatal disc tissue growth and development has not been defined. In the present studies, we generated Runx2 conditional knockout (KO) mice (Runx2(Agc1ER)), in which Runx2 was deleted in Aggrecan-expressing cells in disc tissue at postnatal 2-weeks of age. We then analyzed changes in disc tissue growth and development using histology and immunohistochemical methods in 3-month-old mice. We found that large vacuolated notochordal cells were accumulated in the nucleus pulposus (NP) in Runx2 KO mice. The growth plate cartilage tissue in the disc was thicker in Runx2 KO mice. We also found a significant upregulation of Indian hedgehog (Ihh) expression in the cells in NP cells and in annulus fibrosus cells of Runx2 KO mice. These results demonstrated that Runx2 may play an important role in postnatal disc tissue development through interacting with Ihh signaling.
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