Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 127, Issue 6, Pages 732-737Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2018.12.001
Keywords
Intermediate compounds; Metabolic pathway; Phenol; Specific degradation rate; Resting cells
Funding
- Department of Pharmacology, Yazd University of Medical Sciences, Iran
- Research Division of Tarbiat Modares University (TMU)
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Bisphenol A (BPA) is a toxic compound emitting to the environment mainly by polycarbonate production facilities. In this research, BPA with the initial concentrations in the range of 1-40 mg l(-1) was degraded by Ralstonia eutropha. The bacteria were unable to use BPA as the sole carbon source. Therefore, resting and growing cells of phenol -adapted R. eutropha were used for cometabolic biodegradation of BPA with phenol at the concentration of 100 mg l-1. The optimum initial concentrations of BPA were 20 mg l(-1) in both approaches of cometabolism. By using resting cells, BPA removal efficiency (RE) reached to 57%, however, RE decreased to 37% by growing cells in the presence of phenol. BPA-degrading activity was inhibited at BPA concentrations >20 mg l(-1). Liquid chromatography mass spectrometry technique was used to identify some metabolic intermediates generated during BPA degradation process as 1,2-bis(4-hydroxyphenyl)-2-propanol, 4-(2-propanol)-phenol, 4-hydroxyacetophenone, 4-isopropenylphenol, and 4-hydroxybenzoic acid. Finally, metabolic pathways for BPA degradation were proposed in this study. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.
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