4.6 Article

Integration of diffraction phase microscopy and Raman imaging for label-free morpho-molecular assessment of live cells

Journal

JOURNAL OF BIOPHOTONICS
Volume 12, Issue 4, Pages -

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201800291

Keywords

cellular imaging; label-free imaging; quantitative phase microscopy; Raman spectroscopy

Funding

  1. Chinese University of Hong Kong Direct Research Grant
  2. Shun Hing Institute of Advanced Engineering [BME-p3-18]
  3. Connecticut Children's Innovation Center Seed Money
  4. National Institute of Biomedical Imaging and Bioengineering [2-P41-EB015871-31]
  5. National Institute of General Medical Sciences [DP2GM128198]

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Label-free quantitative imaging is highly desirable for studying live cells by extracting pathophysiological information without perturbing cell functions. Here, we demonstrate a novel label-free multimodal optical imaging system with the capability of providing comprehensive morphological and molecular attributes of live cells. Our morpho-molecular microscopy (3M) system draws on the combined strength of quantitative phase microscopy (QPM) and Raman microscopy to probe the morphological features and molecular fingerprinting characteristics of each cell under observation. While the commonr-path geometry of our QPM system allows for highly sensitive phase measurement, the Raman microscopy is equipped with dual excitation wavelengths and utilizes the same detection and dispersion system, making it a distinctive multi-wavelength system with a small footprint. We demonstrate the applicability of the 3M system by investigating nucleated and nonnucleated cells. This integrated label-free platform has a promising potential in preclinical research, as well as in clinical diagnosis in the near future.

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