Journal
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
Volume 37, Issue 17, Pages 4614-4631Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2018.1557557
Keywords
Sickle hemoglobin; alizarin; spectroscopy; fluorescence; CD; molecular modeling; Hb S polymerization inhibition
Categories
Funding
- DST-SERB [GOI A-738]
- DIC MHRD GOI
Ask authors/readers for more resources
Polymerization of hemoglobin S is a major cause of morbidity and mortality in sickle cell disease, which leads to sickling and destruction of red blood cell. Alizarin, a bioactive compound from Rubia cordifolia, is reported to be blood purifier. This study investigates the potential of alizarin as an antisickling agent, showing a significant decrease in the rate of polymerization, therefore inhibiting the rate of sickling with increasing concentration. Interaction studies indicated that the fluorescence intensity of sickle hemoglobin (Hb S) decreases gradually with increasing alizarin concentration. This suggests the static quenching, where binding constant and the number of binding sites were deduced at different temperatures. The negative values of Gibbs energy change (DG0) strongly suggest that it is entropy-driven spontaneous and exothermic reaction. Negative enthalpy (DH0) and positive entropy (DS0) stipulated that hydrogen and hydrophobic bonding forces were interfering in a hydrophobic micro-environment of b6Val leading to Hb S polymerization inhibition. In circular dichroism (CD) spectra, Hb S in the presence of alizarin shows helical structural changes leading to destabilization of Hb S polymer. These findings were also supported by molecular docking simulation studies using DOCK6 and GROMACS. So, from these findings, we may conclude that alizarin interacts with Hb S through hydrogen bonding and leading to inhibition of Hb S polymerization. Consequently, alizarin may have potential use as an anti-sickle cell medication for sickle cell disorder.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available