Journal
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
Volume 22, Issue 2, Pages 167-178Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10286020.2018.1539080
Keywords
Cordyceps militaris; cordycepin; liquid fermentation; hydrothermal exaction; cost reduction
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In this study, we developed a novel liquid fermentation medium of Cordyceps militaris using pupa powder and wheat bran as nitrogen resources instead of the traditionally used peptone. This process not only reduced the cost by approximately 50%, but increased production by over 30%. Then, we explored a method to extract and purify cordycepin by combining hydrothermal reflux extraction with macroporous resin adsorption, which is inexpensive and suitable for the industrial production. The optimum conditions for hydrothermal reflux were extracting three times at 95 degrees C with 1:10 sample-to-water ratio, and the cordycepin purity with macroporous resin HPD-100 reached 95.23%.
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