Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 47, Pages 12479-12489Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b03703
Keywords
synergistic catabolism; phenylurea herbicide; linuron; amidohydrolase; amidase; bacterial consortium
Funding
- National Natural Science Foundation of China [31670111]
- National Key Research and Development Program [2016YFD0800203]
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Phenylurea herbicides (PHs) are frequently detected as major water contaminants in areas where there is extensive use. In this study, Diaphorobacter sp. strain LR2014-1, which initially hydrolyzes linuron to 3,4-dichloroanaline, and Achromobacter sp. strain ANB-1, which further mineralizes the produced aniline derivatives, were isolated from a linuronmineralizing consortium despite being present at low abundance in the community. The synergistic catabolism of linuron by the consortium containing these two strains resulted in more efficient catabolism of linuron and growth of both strains. Strain LR2014-1 harbors two evolutionary divergent hydrolases from the amidohydrolase superfamily Phh and the amidase superfamily TccA2, which functioned complementarily in the hydrolysis of various types of PHs, including linuron (N-methoxy-N-methyl-substituted), diuron, chlorotoluron, fluomethuron (N,N-dimethyl-substituted), and siduron. These findings show that a bacterial consortium can contain catabolically synergistic species for PH mineralization, and one strain could harbor functionally complementary hydrolases for a broadened substrate range.
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