Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 41, Pages 10760-10768Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b04676
Keywords
elastin hydrolysates; elastase inhibitory activity; molecular docking; fibroblast; antiphotoaging
Funding
- National Key Research and Development Program of China [2017YFD0400200]
- National Postdoctoral Program for Innovative Talents of China [BX201700081]
- China Postdoctoral Science Foundation [2017M622702]
- Guangzhou Science and Technology Plan Project [201604020122]
Ask authors/readers for more resources
This study aimed to investigate the protective effects of bovine elastin hydrolysates on UV-induced skin photoaging in mice and to identify the potent antiphotoaging peptides. Results showed that the ingestion of elastin peptides could obviously ameliorate epidermis hyperplasia and fibroblast apoptosis, and increase the content of hydroxyproline and water in photoaging skin in vivo (p < 0.05). Furthermore, four peptides with elastase inhibitory activity were purified and identified, including GLPY, PY, GLGPGVG, and GPGGVGAL. Interestingly, GLPY and GPGGVGAL exhibited the highest inhibition activity with 58.77% and 42.91% at 10 mM, respectively. This might be attributed to the N-terminal Gly, C-terminal Leu, and Pro at the third position of the N-terminus, which showed stronger affinity and interaction with elastase. Moreover, GLPY and GPGGVGAL could also inhibit the apoptosis of fibroblasts effectively at 50 mu M (p < 0.01). It suggested that elastin peptides had great potential to prevent and regulate skin photoaging.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available