Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 50, Pages 13084-13095Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b04510
Keywords
tea geometrid; pheromone-binding protein; Type II sex pheromone; multispectral and thermodynamic analysis; molecular docking; site-directed mutagenesis
Funding
- National Natural Science Foundation of China [31772544, 31372254]
- Natural Science Foundation of Zhejiang Province [LY18C160004, Y13C140011]
- Science and Technology Plan Project of Hangzhou City [20180432B22]
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Lepidopteran geometrid moth can produce complex Type II sex pheromone components to attract males and trigger mating behavior. Although several sex pheromone components have been identified, it remains unclear whether their physicochemical roles in sex pheromone sensing are the same. Therefore, we utilized tea geometrid (Ectropis obliqua) as an example model to investigate and compare the physicochemical basis of two key Type II sex pheromone components, cis-6,7-epoxy-(3Z,9Z)-3,9-octadecadiene (Z3Z9-6,7-epo-18:Hy) and (Z,Z,Z)-3,6,9-octadecatriene (Z3Z6Z9-18:Hy), interacting with pheromone-binding protein 2 (EoblPBP2) from E. obliqua. Multispectral, thermodynamic, docking, and site-directed mutagenesis indicated that the major sex pheromone component Z3Z9-6,7-epo-18:Hy is more susceptible to pH-tuned than the minor component Z3Z6Z9-18:Hy, whereas Z3Z6Z9-18:Hy seems to be more susceptible to temperature and amino acid mutations than Z3Z9-6,7-epo-18:Hy. Our study suggests that different components of Type II sex pheromone play different binding characters under specific conditions in the physicochemical behavior. This deeply supplements the theoretical knowledge of Type II pheromones involved in the recognition and discrimination in the Lepidopteran sex pheromones family.
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