Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 19, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/ijms19113438
Keywords
soybean; NopP; symbiosis; QTL
Funding
- Ministry of Science and Technology of People's Republic of China Project [2017YFE0111000]
- National Natural Science Foundation of China [31400074, 31471516, 31271747, 30971809]
- Natural Science Foundation of Heilongjiang Province of China [ZD201213]
- Heilongjiang Postdoctoral Science Foundation [LBH-Q16014]
- Harbin Science Technology Project [2013RFQXJ005, 2014RFXXJ012]
- National Key R&D Program of China [2016YFD0100500, 2016YFD0100300, 2016YFD0100201-21]
- Foundation for University Key Teachers from the education department of Heilongjiang Province in China [1254G011]
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In some legume-rhizobium symbioses, host specificity is influenced by rhizobial nodulation outer proteins (Nops). However, the genes encoding host proteins that interact with Nops remain unknown. We generated an Ensifer fredii HH103 NopP mutant (HH103NopP), and analyzed the nodule number (NN) and nodule dry weight (NDW) of 10 soybean germplasms inoculated with the wild-type E. fredii HH103 or the mutant strain. An analysis of recombinant inbred lines (RILs) revealed the quantitative trait loci (QTLs) associated with NopP interactions. A soybean genomic region containing two overlapping QTLs was analyzed in greater detail. A transcriptome analysis and qRT-PCR assay were used to identify candidate genes encoding proteins that interact with NopP. In some germplasms, NopP positively and negatively affected the NN and NDW, while NopP had different effects on NN and NDW in other germplasms. The QTL region in chromosome 12 was further analyzed. The expression patterns of candidate genes Glyma.12g031200 and Glyma.12g073000 were determined by qRT-PCR, and were confirmed to be influenced by NopP.
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