Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 20, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/ijms20010167
Keywords
salt tolerance; RGA1; map-based cloning; iTRAQ; ROS scavenging
Funding
- National Key Research and Development Program of China [2016YFD0100604]
- National Science Foundation of China [31470366]
- Major Special Foundation of Transgenic Plants in China [2018ZX0800913B]
- Fundamental Research Funds for Central Non-profit Scientific Institution [1610392018003]
- Agricultural Science and Technology Innovation Program Cooperation and Innovation Mission [CAAS-XTCX2016001]
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High salinity severely restrains plant growth and results in decrease of crop yield in agricultural production. Thus, it is of great significance to discover the crucial regulators involved in plant salt resistance. Here, we report a novel mutant, sd58, which displays enhanced salt tolerance and dwarf phenotype, by screening from ethyl methane sulfonate (EMS) mutagenized rice mutant library. Genetic analysis showed that sd58 was caused by a single recessive locus. Map-based cloning and allelic test revealed that the phenotypes of sd58 were due to the mutation of RGA1, encoding the alpha subunit of heterotrimeric G protein (G). A point mutation (G to A) was identified at the splicing site (GT-AG) of the first intron in RGA1, which gives rise to the generation of abnormal mRNA splicing forms. Furthermore, 332 differentially abundant proteins (DAPs) were identified by using an Isobaric Tags for Relative and Absolute Quantitation(iTRAQ)-based proteomic technique from seedlings of sd58 and Kitaake in response to salt treatment. Gene Ontology (GO) and KEGG pathway enrichment analysis revealed these proteins were mainly involved in regulation of the processes such as metabolic pathways, photosynthesis and reactive oxygen species (ROS) homeostasis. Under salt stress, sd58 displayed lower ROS accumulation than Kitaake, which is consistent with the higher enzyme activities involved in ROS scavenging. Taken together, we propose that RGA1 is one of the regulators in salt response partially through ROS scavenging, which might be helpful in elucidating salt tolerant mechanisms of heterotrimeric G protein in rice.
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