Journal
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volume 44, Issue 7, Pages 3560-3568Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2018.12.080
Keywords
Electro-conductive carbon cloth; Transgenic Enterobacter aerogenes; Hydrogen fermentation
Categories
Funding
- National Key Research and Development Program-China [2016YFE0117900]
- Zhejiang Provincial Key Research and Development Program-China [2017C04001]
- European Union [797259]
- Marie Curie Actions (MSCA) [797259] Funding Source: Marie Curie Actions (MSCA)
Ask authors/readers for more resources
Long-range extracellular electron transfer through microbial nanowires is critical for efficient bacterial behaviors. The application of carbon cloth on the dark hydrogen fermentation using transgenic Enterobacter aerogenes (E. aerogenes/HoxEFUYH) was first proposed to enhance hydrogen production from glucose. Scanning electron microscopy images showed that the microbial nanowires between E. aerogenes/HoxEFUYH cells almost vanished due to the presence of carbon cloth. Approximately 59.1% of microorganisms concentrated in biofilms on the surface of carbon cloth, which probably promoted the intercellular electron transfer. The results from Fourier transform infrared spectra and Excitation Emission Matrix spectra indicated that carbon cloth biofilms primarily included polysaccharide and protein. Moreover, the fluorophore of biofilms (88.1%) was much higher than that of supernatant (11.9%). The analysis of soluble metabolic degradation byproducts revealed that carbon cloth selectively enhanced the acetate pathway (C6H12O6+ 2H(2)O ->+2CH(3)COOH + 2CO(2) + 4H(2)), but weakened the ethanol pathway (C6H12O6 -> 2C(2)H(5)OH + 2CO(2)). With 1.0 g/L carbon cloth, the hydrogen yield increased by 26.6% to 242 mL/g, and the corresponding peak hydrogen production rate increased by 60.3%. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available