Journal
FRONTIERS IN MATERIALS
Volume 2, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmats.2015.00037
Keywords
SU-8 photoresist; microfluidic devices; capillarity; microfluidic cell culture; hydrogel confinement; self-induced chemotaxis; cell migration
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Funding
- National Research Program of Spain [dpi2011-28262-c04-01, bes-2012-059940]
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Tissues are complex three-dimensional structures in which cell behavior is frequently guided by chemotactic signals. Although starvation and nutrient restriction induce many different chemotactic processes, the recreation of such conditions in vitro remains difficult when using standard cell culture equipment. Recently, microfluidic techniques have arisen as powerful tools to mimic such physiological conditions. In this context, microfluidic three-dimensional cell culture systems require precise control of cell/hydrogel location because samples need to be placed within a microchamber without obstruction of surrounding elements. In this article, SU-8 is studied as structural material for the fabrication of complex cell culture microdevices due to its good mechanical properties and sensor integration capacity. Moreover, SU-8 physical properties and their effect on a successful design for precise control of hydrogel location within microfluidic devices are studied. In particular, this manuscript presents a SU-8 based microdevice designed to create self-induced medium starvation, based on the combination of nutrient restriction and natural cell metabolism. Results show a natural migratory response toward nutrient source, showing how cells adapt to their own microenvironment modifications. The presented results demonstrate the SU-8 potential for microdevice fabrication applied to cell culture.
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