Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 121, Issue -, Pages 691-698Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.10.114
Keywords
Canthaxanthin; Alginate; High methoxyl pectin; Multiple emulsion; External gelation; Functional food
Funding
- National Nutrition and Food Technology Research Institute of Shahid Beheshti University of Medical Sciences
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Canthaxanthin (CX) is an orange-red keto-carotenoid with high antioxidant activity. This functional pigment is sensitive to oxygen, light, pH and heat In this study, CX was produced by the Dietzia natronolimnaea HS-1 and was encapsulated in Alginate (Alg) and Alg-high methoxyl pectin (HMP) through 0/W/0 multiple emulsion/external gelation method to developed resistant microparticles among acidic and neutral pHs. Results showed that initial CX concentration had a significant influence on total CX (TCX), surface CX (SCX), microencapsulation efficiency (EE) and particles size. The highest EE% for Alg (60.21 +/- 0.18) and Alg-HMP (70.60 +/- 0.68) were obtained with CX initial concentration of 11 and 18 g/mg, respectively. Alg microparticles showed smaller size compare to Alg-HMP microcapsules. Presence of CX in microparticles and good antioxidant activity was confirmed by FT-IR spectroscopy and DPPH assay, respectively. CX in vitro release was 66% and 49% in acidic condition and 76% and 50% in neutral condition for Alg and Alg-HMP, respectively. Thus, Alg-HMP-CX18 micro particles were selected to be used in both neutral and acidic foods such as milk and fermented milks products as an antioxidant and a colorant agent. (C) 2018 Published by Elsevier B.V.
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