Journal
ENVIRONMENTAL ENGINEERING RESEARCH
Volume 20, Issue 2, Pages 163-169Publisher
KOREAN SOC ENVIRONMENTAL ENGINEERS
DOI: 10.4491/eer.2014.018
Keywords
Protein solubilization; Response surface methodology; Slaughter blood; Ultrasonification
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Funding
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) [112040-3]
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In this study, we attempted to solubilize protein in slaughter blood (SB) using ultrasonic technology. The application of ultrasonic technology can make enzymatic degradation of SB more effective, which has no comparable alternative for treatment. The SB was homogenized by grinding it for 10 minutes at 10,000 rpm as a pretreatment for preventing its clotting, and then ultrasonic treatment was attempted to solubilize protein in SB. To maximize the efficiency of ultrasonic treatment for SB, the optimum condition of ultrasonic frequency (UF) was determined to be 20 kHz. To optimize the operation conditions of ultrasonification with 20 kHz of frequency, we used response surface methodology (RSM) based on ultrasonic density (UD) and ultrasonification time (UT). The solubilization rate (SR) of protein (%) was calculated to be 101.304 19.4205 X-1 + 0.0398 X-2 + 7.9411 X-1(2) + 0.0001 X-2(2) + 0.0455 X1X2. From the results of the RSM study, the optimum conditions of UD and UT were determined at 0.5 W/mL and 22 minutes, respectively, and SB treated under these conditions was estimated to have a 95% SR. Also, experimentally, a 95.53% SR was observed under same conditions, accurately reflecting the theoretical prediction of 95%.
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