Journal
IMMUNOLOGY AND CELL BIOLOGY
Volume 97, Issue 4, Pages 427-434Publisher
WILEY
DOI: 10.1111/imcb.12231
Keywords
Antitumor T cells; T-cell development; T-cell receptor; T-cell selection
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Funding
- Canadian Institutes of Health Research [FND-154332]
- Terry Fox Foundation [TFF-15005]
- Krembil Foundation
- Canada Research Chair in Developmental Immunology
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The differentiation of human hematopoietic stem cells into CD8 T cells can be achieved in vitro with the OP9-DL4 system. We considered that in the absence of medullary thymic epithelial cells, which serve to restrict the breath of the T-cell receptor (TCR) repertoire by expressing tissue-restricted antigens, a distinct repertoire would be generated in vitro. To test this notion, we compared the TCR-V alpha/V beta (TRAV/TRBV) gene usage of major histocompatibility complex-restricted antigen (MART-1)-specific T cells generated in vitro to that from ex vivo naive T cells and tumor-infiltrating lymphocytes (TILs) using high-throughput DNA sequencing. In contrast to naive T cells and TILs, which showed the expected narrow TRAV repertoire, in vitro-generated MART-1-specific T cells used almost all TRAV gene families and displayed unique CDR3 lengths. Our work demonstrates that the OP9-DL4 system supports the creation of a broad antigen-specific TCR repertoire, suggesting that T cells generated in vitro may undergo a different set of selection events that otherwise constrains the TCR repertoire of thymus-derived T cells.
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