Journal
IMMUNITY
Volume 49, Issue 4, Pages 725-+Publisher
CELL PRESS
DOI: 10.1016/j.immuni.2018.08.015
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Funding
- CHOA
- Emory Pediatrics
- URMC Flow Cytometry Cores
- National Institutes of Health [T32-DK007656, AR043737, AR069572, U19-A1110483, R37-A1049660, P01-A1125180, P01-A11078907, R01-A1110508, R01-A1121252, U19-A1109962]
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Systemic Lupus Erythematosus (SLE) is characterized by B cells lacking IgD and CD27 (double negative; DN). We show that DN cell expansions reflected a subset of CXCR5(-) CD11c(+) cells (DN2) representing pre-plasma cells (PC). DN2 cells predominated in African-American patients with active disease and nephritis, anti-Smith and anti-RNA autoantibodies. They expressed a T-bet transcriptional network; increased Toll-like receptor-7 (TLR7); lacked the negative TLR regulator TRAF5; and were hyper-responsive to TLR7. DN2 cells shared with activated naive cells (aNAV), phenotypic and functional features, and similar transcriptomes. Their PC differentiation and autoantibody production was driven by TLR7 in an interleukin-21 (IL-21)-mediated fashion. An in vivo developmental link between aNAV, DN2 cells, and PC was demonstrated by clonal sharing. This study defines a distinct differentiation fate of autoreactive naive B cells into PC precursors with hyper-responsiveness to innate stimuli, as well as establishes prominence of extra-follicular B cell activation in SLE, and identifies therapeutic targets.
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