Journal
HUMAN GENETICS
Volume 138, Issue 1, Pages 1-19Publisher
SPRINGER
DOI: 10.1007/s00439-018-1953-5
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Funding
- Russian Science Foundation [17-75-20095]
- Russian Academy of Sciences
- state assignment of FASO Russia
- Russian Science Foundation [17-75-20095] Funding Source: Russian Science Foundation
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Despite the recent discover of genome-editing methods, today we can say these approaches have firmly entered our life. Two approachesknocking out malfunctioning gene allele or correcting the mutation with precise knock-incan be used in hereditary monogenic diseases treatment. The latter approach is relatively ineffective. Modern data about the ways of repair of double-strand DNA breaks formed by nucleases are presented in this review. The main part of the review is devoted to the ways of increasing precise and effective knock-in: inhibition of non-homologous end joining and stimulation of homology-directed repair key factors, use of small molecules with unknown mechanism of action, cell-cycle synchronization and cell-cycle-dependent activity of Cas9, donor molecule design, selection, alternative methods for insertion and other approaches.
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