Journal
FUTURE MICROBIOLOGY
Volume 14, Issue 1, Pages 11-21Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2018-0278
Keywords
PafA; PafB; PafC; proteasome; proteolysis; Pup
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Funding
- Israel Science Foundation [587/17]
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Aim:Mycobacterium tuberculosis possesses an intracellular tagging and degradation system, which has emerged as a target for development of anti-tuberculosis agents. In this system, PafA is the ligase that marks proteins for degradation by their covalent modification with a protein modifier. Here, we studied pafA transcriptional regulation, which remained elusive despite its importance for M. tuberculosis virulence. Materials & methods: Working with Mycobacterium smegmatis, a mycobacterial model organism, we examined the involvement of the global regulators PafB and PafC in pafA regulation. Results: PafBC activated pafA transcription following DNA damage, resulting in efficient cellular recovery. Conclusion: The results unraveled the involvement of PafBC in pafA transcription, and revealed the importance of proper PafA regulation in mycobacterial physiology.
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