Species-specific peptide-based liquid chromatography-mass spectrometry monitoring of three poultry species in processed meat products

The detection of adulteration and mislabeling of food products, including intensively processed meat, is a challenge which needs urgent solutions to protect consumers' rights. The aim of the study was to demonstrate the feasibility of species-specific peptide-based LC-MS methods for monitoring duck, goose and chicken in processed meat products. Food commodities of various compositions, subjected to various treatments, including homogenization, cooking, roasting, drying, and sterilization during production, were examined to ensure that MS-based methods are resistant to matrix composition changes. A qualitative LC-QQQ multiple reaction monitoring (MRM) method was developed which allows high-confidence monitoring of duck, goose and chicken meat (ten specific peptides), simultaneously with beef and pork (seven peptides), in the presence of turkey meat, in highly processed food. The developed LC-MS methods can be used for food authentication, monitoring of the food composition conformity with label statements and detection of adulteration of poultry-containing food products.