Journal
FOOD CHEMISTRY
Volume 272, Issue -, Pages 58-65Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.08.021
Keywords
Au@CQDs; Melamine; Fluorescence standard array; Smartphone; Gold nanoparticles; Carbon quantum dots
Funding
- National Natural Science Foundation of China, China [31671844]
- National Key Research and Development Program of China, United States [2016YFD0401104]
- Natural Science Foundation of Jiangsu Province, China [BK20160506, BE2016306]
- International Science and Technology Cooperation Project of Jiangsu Province, China [BZ2016013]
- Six Talent Peaks Project in Jiangsu Province, China [GDZB-016]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Gold nanoparticles@Carbon quantum dots nanocomposites (Au@CQDs) were designed for analyzing melamine in milk visually. Fluorescent emission of Au@CQDs enhanced with the increase of melamine concentration. A calibration curve (R-2 = 0.9856) and fluorescence standard array were established for detection of melamine in the range of 1 mu M-10 mu M. Limit of quantification and limit of detection were 12 nM and 3.6 nM, respectively. The approximate concentration of melamine adulterated in milk samples were detected visually by the fluorescence standard array and smartphone. The accurate concentration was obtained by the calibration curve and portable fluorescence spectrometer with recoveries of 105.64%-102.75% and less than 2% of coefficient of variation (CV) (n = 18). This performance was consistent with that of the high-performance liquid chromatography (HPLC) method (recoveries of 105.56%-107.95% with 2.24%-3.56% of CVs). The Au@CQDs-based fluorescent method for visual and accurate detection of melamine in milk was presented and showed strong application potential.
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