Journal
FOOD CHEMISTRY
Volume 270, Issue -, Pages 47-52Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.07.069
Keywords
LC-MS/MS; Antioxidants; Cucumber; Nanoparticle; Extraction; Metabolites
Funding
- National Science Foundation (NSF, Australia)
- U.S. Environmental Protection Agency (EPA, Ireland) [NSF-EF0830117]
- Agilent Technologies
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Targeted metabolomics aims to provide a new approach to investigate metabolites and gather both qualitative and quantitative information. We describe a protocol for extraction and analysis of plant metabolites, specifically 13 secondary metabolites (antioxidants) using liquid chromatography coupled to triple quadrupole mass spectrometry (LC-MS/MS), with high linearity (R2 > 0.99) and reproducibility (0.23-6.23 R%) with low limits of detection (> 0.001 ng/mL) and quantification (> 0.2 ng/mL). The protocol was applied to study the antioxidant response of cucumber plants exposed to nanocopper pesticide. Dose-dependent changes in antioxidant concentrations were found, and 10 antioxidants were significantly consumed to scavenge reactive oxygen species, protecting plants from damage. Levels of three antioxidants were up-regulated, as a response to the depletion of the other antioxidants, signaling activation of the defense system. We demonstrated that the reported LC-MS/MS method provides a quantitative analysis of antioxidants in plant tissues, for example to investigate interactions between plants and nanomaterials.
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