Journal
FOOD CHEMISTRY
Volume 270, Issue -, Pages 17-24Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.07.056
Keywords
Aroma; Camellia sinensis; Phenylalanine; Phenylpyruvic acid; Tea; Volatile
Funding
- National Natural Science Foundation of China [31601787]
- Guangdong Natural Science Foundation [2016A030313652, 2016A030306039]
- Guangdong Innovation Team of Modern Agricultural Industry Technology System [2017LM1143]
- Guangdong Special Support Plan for Training High-Level Talents [2016TQ03N617]
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Aromatic aroma compounds contribute to flavor of tea (Camellia sinensis (L.) O. Kuntze) and they are mostly derived from L-phenylalanine via trans-cinnamic acid or directly from L-phenylalanine. The objective of this study was to investigate whether an alternative pathway derived from L-phenylalanine via phenylpyruvic acid is involved in formation of aroma compounds in tea. Enzyme reaction with phenylpyruvic acid showed that benzaldehyde, benzyl alcohol, and methyl benzoate were derived from phenylpyruvic acid in tea leaves. Feeding experiments using [H-2(8)] L-phenylalanine indicated that phenylpyruvic acid was derived from L-phenylalanine in a reaction catalyzed by aromatic amino acid aminotransferases (AAATs). CsAAAT1 showed higher catalytic efficiency towards L-phenylalanine (p <= 0.001) while CsAAAT2 showed higher catalytic efficiency towards L-tyrosine (p <= 0.001). Both CsAAATs were localized in the cytoplasm of leaf cells. In conclusion, an alternative pathway for the formation of aromatic aroma compounds derived from L-phenylalanine via phenylpyruvic acid occurred in tea leaves.
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