Journal
FOOD CHEMISTRY
Volume 271, Issue -, Pages 767-772Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.07.143
Keywords
Pentaplex PCR; Direct PCR; Meat species; Identification
Funding
- Faculty of Science, Prince of Songkla University, Thailand [156004]
Ask authors/readers for more resources
A direct pentaplex PCR assay was developed for the identification of meat from sources other than those declared on the packaging. Species-specific primers were designed, based on the mitochondrial cytochrome oxidase I (COI) gene. The assay amplified specific DNA fragments from dog (230 bp), duck (283 bp), buffalo (363 bp), goat (396 bp), and sheep (477 bp). The proposed method is capable of identifying target species accurately and is reproducible, sensitive and robust for use with real-world foods and food products. In total, 26 of 117 meat and commercial food products tested were shown to contain DNA from species not declared on the label.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available