Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 84, Issue -, Pages 816-824Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2018.10.064
Keywords
Aeromonas hydrophila; Transcriptome; Signaling pathway; Toll-like receptor (TLR); Interferon regulatory factor (IRF)
Funding
- Two Sides Supporting Plan in Sichuan Agricultural University, PR China [00770103]
- Basic Research Program of Yunnan Province, PR China [2016FA044, 2017FB028]
- National Nature Science Foundation of China (NSFC), PR China [U1702233, 31672282]
- Applied Basic Research from Technological Office of Sichuan Province, PR China [2015JY0206]
- Yunnan social development of science and technology plan, PR China [2016ZA004]
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Schizothorax prenatal (S. prencmd), an important species of economical fish in Southwest China, is susceptible to Aeromonas hydrophila (Ah). To understand the immune response to Ah, the transcriptome profiling of spleen of S. prenanti was analyzed after Ah infection. A total of 6, 213 different expression genes (DEGs) were obtained, including 3, 066 up-regulated DEGs and 3, 147 down-regulated DEGs. These DEGs were annotated by KEGG and GO databases, so that the immune-related DEGs (IRDs) can be identified and classified. Then, the interesting IRDs were screened to build heat map, and the reliability of the transcriptome data was validated by qPCR. In order to clarify the mechanism of signal transduction in the anti-bacterial immunity, the signaling pathway initiated by TLRs was predicted. In this pathway, TLR25 and TLR5 mediate the NF-kappa B and AP-1 signals via MyD88-dependent pathway. Meanwhile, the type I IFN (IFN alpha/beta) induced by IRF1 and IRF3/7 may play an important role in the anti-bacterial immunity. In conclusion, this study preliminarily provides insights into the mechanism of signal transduction after Ah infection in S. prenanti, which contributes to exploring the complex anti-bacterial immunity.
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