Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 84, Issue -, Pages 906-911Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2018.10.080
Keywords
Bursicon; Homodimer; Anti-microbial peptide (AMP); Bacteriostasis; Innate immune
Funding
- Innovation Team of Tianjin Fisheries Research System [ITTFRS2017007]
- Tianjin Development Program for Innovation and Entrepreneurship [TD13-5076]
- Natural Science Foundation of Tianjin [15JCYJC51400]
Ask authors/readers for more resources
Bursicon is a neurohormone belonging to the cystine knot protein family. It consists of two subunits (burs a and burs beta) and plays a pivotal role in cuticle tanning and wing expansion in insects. Recent studies show that homologous crustacean bursicon stimulates cuticle thickening and granulation of hemocytes in the crab Callinectes sapidus. Here we investigate whether bursicon homodimers function in immunoprotective defense systems of shrimp. We found that abdominal ganglion was the main neurohemal release site of bursicon in Neocaridina heteropoda. Bacterial infections induced overexpression of burs alpha (bursicon alpha) and burs beta (bursicon beta). RNAi of burs alpha, burs beta or both inhibited the expression of anti-microbial peptide (AMP) genes. Treating shrimp adults with r-bursicon (recombinant bursicon) homodimers led to up-regulation of three AMP genes. Besides, through the induced AMPs, r-bursicon homodimers enhanced the bacteriostasis of shrimp in vivo and in vitro. These findings demonstrate a novel function of bursicon in crustacean that it induces innate immune via up-regulating the expression of genes encoding AMPs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available