Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 84, Issue -, Pages 627-638Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2018.09.052
Keywords
Ionic liquids; Silver carp; Oxidative stress; p38MAPK/NF-kappa B; microRNAs; Inflammatory response
Funding
- National Natural Science Foundation of China [31702349, 31472285]
- Key Subjects of Biology in Henan Province, China
Ask authors/readers for more resources
The present study aimed to determine the chronic toxicity of 1-methyl-3-octylimidazolium bromide ([Cgmim]Br) on the silver carp to further reveal the toxicological mechanisms of ionic liquids. Chronic exposure of silver carp to (C(8)mim)Br at concentrations of 1.095 and 4.380 mg/L for 60 d was conducted under laboratory conditions. The results revealed that chronic exposure to [C(8)mim]Br inhibited the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and reduced glutathione (GSH) levels while markedly increasing malondialdehyde (MDA) and protein carbonyl (PC) levels in fish spleen, indicating that [Csmim]Br treatment induced oxidative stress. Additionally, long-term exposure to [C(8)mim]Br markedly upregulated the expressions of nuclear factor-kappa Beta (NF-kappa Beta), inducible nitric oxide synthase (iNOS), interleukin-1 beta (IL-1 beta), IL-6, tumour necrosis factor-alpha (TNF-alpha), and interferon-y (IFN-gamma); altered the levels of transforming growth factor-beta (TGF-beta); and increased the mRNA levels of p38MAPK, c-fos, c-jun, and c-myc, suggesting that long-term exposure to [C(8)mim]Br might promote the inflammatory response in fish spleen and that p38MAPK/NF-kappa Beta signalling may potentially be involved in this process. Moreover, [Csmim]Br-exposure altered lysozyme activity and complement 3 (C3) and immunoglobulin M (IgM) content, indicating that chronic [C(8)mim]Br exposure also has immunotoxic effects on silver carp. Furthermore, we also found that [C(8)mim]Br exposure reduced miR-125b levels, altered miR-143 levels, and upregulated miR-155 and miR-21 levels, suggesting that these miRNAs may be involved in the [C(8)mim]Br-induced inflammatory response in fish spleen. In summary, the present study indicates that chronic exposure to [C(8)mim]Br induces inflammation in fish spleen and that oxidative stress mediated p38MAPK/NF-kappa Beta signalling and miRNAs may play a key role in this process.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available