Journal
FEBS LETTERS
Volume 593, Issue 3, Pages 319-338Publisher
WILEY
DOI: 10.1002/1873-3468.13311
Keywords
affinity purification; Arabidopsis thaliana; circadian rhythms; flowering time; quantitative mass spectrometry
Funding
- Wellcome Trust [096995/Z/11/Z]
- BBSRC
- EPSRC [BB/N005147/1, BB/D019621, BB/J009423]
- NIH [GM079712]
- NIH/NIGMS [P41 GM103533]
- Next-Generation BioGreen 21 Program grant (SSAC, Rural Development Administration, Republic of Korea) [PJ013386]
- BBSRC [BB/N012348/1, BB/N005147/1, BB/D019621/1] Funding Source: UKRI
- Wellcome Trust [096995/Z/11/Z] Funding Source: Wellcome Trust
Ask authors/readers for more resources
The plant-specific protein GIGANTEA (GI) controls many developmental and physiological processes, mediating rhythmic post-translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI's multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time-specific manner, using proteomics on Arabidopsis plants expressing epitope-tagged GI. We detected previously identified (in)direct interactors of GI, as well as proteins implicated in protein folding, or degradation, and a previously uncharacterized transcription factor, CYCLING DOF FACTOR6 (CDF6). We verified CDF6's direct interaction with GI, and ZEITLUPE/FLAVIN-BINDING, KELCH REPEAT, F-BOX 1/LIGHT KELCH PROTEIN 2 proteins, and demonstrated its involvement in photoperiodic flowering. Extending interaction proteomics to time series provides a data resource of candidate protein targets for GI's post-translational control.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available