Journal
FEBS JOURNAL
Volume 286, Issue 1, Pages 139-150Publisher
WILEY
DOI: 10.1111/febs.14700
Keywords
DDX47; FANCD2; hnRNP U; replication stress; R-loops
Categories
Funding
- JSPS KAKENHI Grant [JP23114010, JP26550026, JP15H01738, JP26281021]
- Uehara Memorial Foundation
- Astellas Foundation for Research on Metabolic Disorders
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
Ask authors/readers for more resources
R-loops, which consist of DNA : RNA hybrids and displaced single-strand DNA, are a major threat to genome stability. We have previously reported that a key Fanconi anemia protein, FANCD2, accumulates on large fragile genes during mild replication stress in a manner depending on R-loops. In this study, we found that FANCD2 suppresses R-loop levels. Furthermore, we identified FANCD2 interactions with RNA processing factors, including hnRNP U and DDX47. Our data suggest that FANCD2, which accumulates with R-loops in chromatin, recruits these factors and thereby promotes efficient processing of long RNA transcripts. This may lead to a reduction in transcription-replication collisions, as detected by PLA between PCNA and RNA Polymerase II, and hence, lowered R-loop levels. We propose that this mechanism might contribute to maintenance of genome stability during mild replication stress.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available