Journal
ENGINEERING
Volume 1, Issue 2, Pages 269-274Publisher
ELSEVIER SCIENCE BV
DOI: 10.15302/J-ENG-2015062
Keywords
MCF-7 cellular spheroids; bioprinting; hydrogels; concave wells; tissue on a chip
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Funding
- National Natural Science Foundation of China [11372243, 11532009, 11522219]
- China Postdoctoral Science Foundation [2013M540742]
- Doctoral Program of Higher Education of China [20130201120071]
- Natural Science Basic Research Plan in Shaanxi Province of China [2014JQ1004]
- Fundamental Research Funds for the Central Universities
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Cellular spheroids serving as three-dimensional (3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cellembedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening.
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