Journal
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
Volume 63, Issue -, Pages 38-44Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2015.09.021
Keywords
Hedera plant; SRAP molecular marker; Cluster analysis; HPLC detection; Medicinal value
Funding
- Education Department of the Hunan Province project [15A089]
- Graduate Innovative Projects in Hunan Province [CX2014E290]
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Hedera plants are ornamental vines which are regarded as traditional medicinal plants. These plants are widely cultivated worldwide; moreover, new species and varieties have been discovered. For the first time, molecular markers based on sequence-related amplified polymorphism (SRAP) were used in this study to classify species and to analyse the genetic relationship of 21 Hedera plant varieties which are commonly cultivated in China. We also employed high-performance liquid chromatography (HPLC) to evaluate the medicinal value of these plants by measuring their hederacoside C contents. Results of SRAP -PCR and cluster analyses show that the SRAP molecular markers can successfully be applied in Hedera plants and are available. In addition, HPLC detection results indicate that the Chinese wild species in group I contain less than 30 mg/g hederacoside C and do not comply with the European Pharmacopoeia standards. By contrast, groups II and III exhibit high medicinal value and accord with these standards. (C) 2015 Elsevier Ltd. All rights reserved.
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