From conjugation to T4S systems in Gram-negative bacteria: a mechanistic biology perspective

Conjugation is the process by which bacteria exchange genetic materials in a unidirectional manner from a donor cell to a recipient cell. The discovery of conjugation signalled the dawn of genetics and molecular biology. In Gram-negative bacteria, the process of conjugation is mediated by a large membrane-embedded machinery termed conjugative type IV secretion (T4S) system, a large injection nanomachine, which together with a DNA-processing machinery termed the relaxosome and a large extracellular tube termed pilus orchestrates directional DNA transfer. Here, the focus is on past and latest research in the field of conjugation and T4S systems in Gram-negative bacteria, with an emphasis on the various questions and debates that permeate the field from a mechanistic perspective.