Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 167, Issue -, Pages 29-35Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2018.09.120
Keywords
Lipid accumulation; Liver; Rana nigromaculata; PFOA; PPAR gamma
Categories
Funding
- Natural Science Foundation of Zhejiang Province of China [LY15C030007]
- Excellent Talent Innovation Experiment Program for University Students in China [201710346009]
- Guangzhou Key Laboratory of Environmental Exposure and Health [GZKLEEH201601]
- Program for 151 Talents in Zhejiang Province [4108Z061700303]
- Program for 131 Talents in Hangzhou City [4105F5061700104]
- Excellent Talent Program in Hangzhou Normal University national innovation experiment program for university students [201710346009]
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Perfluorooctanoic acid (PFOA) is a perfluorinated compound that is widely distributed, is persistent in the environment, and has a low-level chronic exposure effect on human health. The aim of this study was to investigate the peroxisome proliferator activated receptors gamma (PPAR gamma) and the sterol regulatory element-binding protein 2 (SREBP2) signaling pathways in regulating the lipid damage response to PFOA in the livers of amphibians. Male and female frogs (Rana nigromaculata) were exposed to 0, 0.01, 0.1, 0.5 and 1 mg/L PFOA. After treatment, we evaluated the pathological changes in the liver by Oil Red Or staining and examined the total cholesterol (T-CHO) and triglyceride (TG) contents. The mRNA expression levels of PPARy, Fatty acid synthase (FAS), Acetyl-CoA carbwcylase (ACC), Glycerol-3-phosphate acyltransferase (GPAT), SREBP2 and 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The administration of PFOA caused marked lipid accumulation damage in the amphibian livers. The T-CHO contents were elevated significantly after PFOA treatment; these results show a dose-dependent manner in both sexes. The TG content showed a significant increase in male livers, while it was elevated significantly in female livers. The RT-PCR results showed that the mRNA expression levels of PPAR gamma, ACC, FAS, GPAT, SREBP2 and HMG-CoA were significantly dose-dependently increased in the PFOA-treated groups compared with those of the control group. Our results demonstrated that PFOA-induced lipid accumulation also affected the expression levels of genes FAS, ACC, GPAT and HMG-CoA in the PPARy and SREBP2 signaling pathways in the liver. These finding will provide a scientific theoretical basis for the protection of Rana nigromaculata against PFOA effects.
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