Journal
DYES AND PIGMENTS
Volume 160, Issue -, Pages 48-57Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2018.07.036
Keywords
Cysteine; Fluorescent probe; Michael addition; Imidazo[1,5-alpha]pyridine; Cell imaging
Funding
- National Natural Science Foundation of China [31471693]
- Jiangsu Provincial Policy Guidance Plan (university-industry-research cooperation) prospective joint research projects [BY2016005-07]
- Jiangsu Agricultural Science and Technology Innovation Fund [CX(17)3052]
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A new imidazo [1,5-alpha]pyridine-based fluorescent probe, named MZC-AC, is developed for the detection of cysteine (Cys) based on an excited state intramolecular proton transfer (ESIPT) mechanism. The probe MZC-AC uses an acrylate moiety as the ESIPT blocking group as well as the recognition site. Upon the treatment with Cys, MZC-AC exhibits a dramatic fluorescence enhancement (85-fold) and a large Stokes shift (166 nm). The limit of detection obtained from fluorescent titration was as low as 0.07 mu M. MZC-AC displays highly sensitive and selective response to Cys over homocysteine (Hcy) and glutathione (GSH). Significantly, MZC-AC could be used to detect Cys in living cells.
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