Journal
DEVELOPMENT
Volume 145, Issue 21, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.166728
Keywords
Zebrafish; Gastrulation; Tailbud; Axial elongation
Categories
Funding
- Henry Dale Fellowship - Wellcome Trust [109408/Z/15/Z]
- Henry Dale Fellowship - Royal Society [109408/Z/15/Z]
- Erasmus+ Traineeship scheme of the European Commission
- Engineering and Physical Sciences Research Council [EP/R025398/1]
- British Society of Developmental Biology/The Company of Biologists/Gurdon Summer Studentship
- Max-Planck-Gesellschaft
- European Research Council (CoG SmartMic) [647885]
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek TOP award [714.016.001]
- Foundation for Fundamental Research on Matter - Nederlandse Organisatie voor Wetenschappelijk Onderzoek [FOM-14NOISE01]
- KWF Kankerbestrijding
- European Research Council [ERC-AdG 742225-IntScOmics]
- EPSRC [EP/R025398/1] Funding Source: UKRI
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During gastrulation, embryonic cells become specified into distinct germ layers. In mouse, this continues throughout somitogenesis from a population of bipotent stem cells called neuromesodermal progenitors (NMps). However, the degree of self-renewal associated with NMps in the fast-developing zebrafish embryo is unclear. Using a genetic clone-tracing method, we labelled early embryonic progenitors and found a strong clonal similarity between spinal cord and mesoderm tissues. We followed individual cell lineages using light-sheet imaging, revealing a common neuromesodermal lineage contribution to a subset of spinal cord tissue across the anterior-posterior body axis. An initial population subdivides at mid-gastrula stages and is directly allocated to neural and mesodermal compartments during gastrulation. A second population in the tailbud undergoes delayed allocation to contribute to the neural and mesodermal compartment only at late somitogenesis. Cell tracking and retrospective cell fate assignment at late somitogenesis stages reveal these cells to be a collection of mono-fated progenitors. Our results suggest that NMps are a conserved population of bipotential progenitors, the lineage of which varies in a species-specific manner due to vastly different rates of differentiation and growth.
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