Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 173, Issue -, Pages 689-697Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2018.10.053
Keywords
Mecobalamin; Aligned; Silk fibroin; Schwann cell; Tissue regeneration
Funding
- National Key Research and Development Program of China [2017YFA0701304, 2016YFC1101600]
- National Natural Science Foundation of China [51603106, 81671823, 31771054]
- Jiangsu Overseas Research & Training Program for University Prominent Young & Middle-aged Teachers and Presidents
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Suitable micro/nanofibers orientation in tissue engineered scaffold could guide cells outgrowth and migration during tissue regeneration. However, it is still a challenge to prepare a simple, high-strength and aligned fibers scaffold. Herein, we report a facile strategy to construct high-strength silk fibroin (SF) scaffolds with aligned fibers orientation by crosslinking aligned SF fibers with regenerated SF solution for guiding neuronal growth direction. The fibers could be easily controlled within the same orientation. The aligned fibers and protein protein interfacial bonding between SF fibers and regenerated SF solution reinforced the mechanical properties to form a high-strength scaffold. Schwann cells and dorsal root ganglion (DRG) neuron could migrate along with the uniform orientation of scaffolds. Mecobalamin was loaded into aligned SF scaffold to promote neurite growth and neuron survival through methylation cycle by activation of Erk1/2 and Akt. Additionally, mecobalamin loaded aligned SF scaffold demonstrated good biocompatibility, inflammatory cells also showed decreased profiles with time extensions. This facile strategy could enrich the fabrication methods to prepare aligned fibers scaffolds in the tissue engineering.
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