Journal
MICROBIAL BIOTECHNOLOGY
Volume 9, Issue 4, Pages 496-501Publisher
WILEY-BLACKWELL
DOI: 10.1111/1751-7915.12314
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Funding
- Biotechnology and Biological Sciences Research Council [BB/J004529/1]
- Turkish Ministry of Education
- Biotechnology and Biological Sciences Research Council [BBS/E/F/00044453] Funding Source: researchfish
- BBSRC [BBS/E/F/00044453] Funding Source: UKRI
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Lactobacillus johnsonii FI9785 has an eps gene cluster which is required for the biosynthesis of homopolymeric exopolysaccharides (EPS)-1 and heteropolymeric EPS-2 as a capsular layer. The first gene of the cluster, epsA, is the putative transcriptional regulator. In this study we showed the crucial role of epsA in EPS biosynthesis by demonstrating that deletion of epsA resulted in complete loss of both EPS-1 and EPS-2 on the cell surface. Plasmid complementation of the epsA gene fully restored EPS production, as confirmed by transmission electron microscopy and nuclear magnetic resonance (NMR) analysis. Furthermore, this complementation resulted in a twofold increase in the expression levels of this gene, which almost doubled amounts of EPS production in comparison with the wild-type strain. Analysis of EPS by NMR showed an increased ratio of the heteropolysaccharide to homopolysaccharide in the complemented strain and allowed identification of the acetylated residue in EPS-2 as the (1,4)-linked Glcp unit, with the acetyl group located at O-6. These findings indicate that epsA is a positive regulator of EPS production and that EPS production can be manipulated by altering its expression.
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