Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 25, Issue 3, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cmi.2018.11.004
Keywords
Antimicrobial resistance; Carbapenemase; Healthcare-associated infections; IncF; Outbreak
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Funding
- Ministry of Health of Italy [RF-2011-02346987]
- Fondo ricerca e giovani 2017 of the University of Pavia
- Instituto de Salud Carlos III Subprograma Estatal de Movilidad, MAES [MV17/00046]
- Sara Borrell contract [CD15/00017]
- FAPESP [2017/16754-5]
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Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying blaNDM-1 or blaOXA-232 carbapenemase genes were assembled and sequences were analysed. Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying blaOXA-232 and IncF plasmids carrying blaNDM-1. The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide. Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone. (c) 2018 Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.
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