Journal
CHINESE CHEMICAL LETTERS
Volume 30, Issue 1, Pages 99-102Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2018.10.029
Keywords
Supercritical fluid extraction; Liquid chromatography-mass spectrometry; Column-switching system; Capsaicin
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Funding
- National Natural Science Foundation of China [21621003]
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A novel on-line system composed of supercritical fluid extraction (SFE), dilution line and reverse phase liquid chromatography/mass spectrometry (RPLC/MS) was constructed for on-line extraction and reverse phase separation of some fat-soluble components in foods. Three columns including a trap column, concentration column and analytical column were used for trapping the fat-soluble components, on-line enrichment and reverse phase separation, respectively. Capsaicinoids were on-line extracted by a CO2 supercritical fluid, then concentrated and separated by using the C-18 columns, finally detected by mass spectrometry (MS). Capsaicin eluted at 10.1 min and limit of detection (LOD, S/N = 3) for the standard solution is 0.55 pg. The linearity was calculated with a value of coefficient of determination (R-2) >= 0.998 in the range of 1.1-8.5 ng. Concentrations of capsaicin in the green, yellow, and red bell peppers were determined to be 60.33 ng/g, 31.79 ng/g, 35.38 ng/g, respectively. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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