Journal
MICROSYSTEMS & NANOENGINEERING
Volume 1, Issue -, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/micronano.2015.2
Keywords
MEMS; implantable microelectrode array; glutamate; electrophysiological detection; in vivo
Funding
- Major National Scientific Research Plan [2011CB933202, 2014CB744605]
- NSFC [61125105, 61471342]
- Beijing Science and Technology Plan [Z141100000214002, Z141102003414014]
- Key Programs of the Chinese Academy of Sciences [KJZD-EW-L11-2]
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L-glutamate, the most common excitatory neurotransmitter in the mammalian central nervous system (CNS), is associated with a wide range of neurological diseases. Because neurons in CNS communicate with each other both electrically and chemically, dual-mode (electric and chemical) analytical techniques with high spatiotemporal resolution are required to better understand glutamate function in vivo. In the present study, a silicon-based implantable microelectrode array (MEA) composed of both platinum electrochemical and electrophysiological microelectrodes was fabricated using micro-electromechanical system. In the MEA probe, the electrophysiological electrodes have a low impedance of 0.018 M Omega at 1 kHz, and the electrochemical electrodes show a sensitivity of 56 pA mu M-1 to glutamate and have a detection limit of 0.5 mu M. The MEA probe was used to monitor extracellular glutamate levels, spikes and local field potentials (LFPs) in the striatum of anaesthetised rats. To explore the potential of the MEA probe, the rats were administered to KCl via intraperitoneal injection. K+ significantly increases extracellular glutamate levels, LFP low-beta range (12-18 Hz) power and spike firing rates with a similar temporal profile, indicating that the MEA probe is capable of detecting dual-mode neuronal signals. It was concluded that the MEA probe can help reveal mechanisms of neural physiology and pathology in vivo.
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