Journal
CHEMISTRY AND PHYSICS OF LIPIDS
Volume 219, Issue -, Pages 15-22Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2019.01.007
Keywords
Escherichia coli; Ion mobility; Mass spectrometry; Lipidomics; Phospholipids
Categories
Funding
- UW Royalty Research Fund [A128444]
- National Institutes of Health [R01A1136979]
- University of Washington (UW) School of Pharmacy Faculty Innovation Fund
- Department of Medicinal Chemistry in the School of Pharmacy at the UW
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Our understanding of phospholipid biosynthesis in Gram-positive and Gram-negative bacteria is derived from the prototypical Gram-negative organism Escherichia coli. The inner and outer membranes of E. coli are largely composed of phosphatidylethanolamine (PE), minor amounts of phosphatidylglycerol (PG) and cardiolipin (CL). We report here the utility of hydrophilic interaction liquid chromatography (HILIC) paired with ion mobility mass spectrometry (IM-MS) for the comprehensive analysis of the E. coli lipidome. Using strains with chromosomal deletions in the PG and CL synthesis genes pgsA and cisABC, respectively, we show that defective phospholipid biosynthesis in E. colt results in fatty-acid specific changes in select lipid classes and the presence of the minor triacylated phospholipids, acylphosphatidyl glycerol (acylPG) and N-acylphosphatidylethanolamine (N-acylPE). Notably, acylPGs were accumulated in the cIsABC-KO strain, but were absent in other mutant strains. The separation of 1-lyso and 2-lyso-phosphatidylethanolamines (lysoPEs) is demonstrated in both the HILIC and IM dimensions. Using our previously validated calibration method, collision cross section values of nearly 200 phospholipids found in E. toll were determined on a traveling wave IM-MS platform, including newly reported values for cardiolipins, positional isomers of lysoPEs, acylPGs and N-acylPEs.
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