Journal
CELL METABOLISM
Volume 29, Issue 4, Pages 950-+Publisher
CELL PRESS
DOI: 10.1016/j.cmet.2018.11.013
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Funding
- National Natural Science Foundation of China [81570093, 81422001, 81370654, 31722033, 91649123, 31671484]
- NSFC [81721004]
- National Basic Research Program of China (973 Program) [2014CB965000, 2015CB910403, 2017YFA050400, 2017YFC0906900]
- 1,000-Youth Elite Program
- Natural Science Foundation of Shanghai [17ZR1415500]
- National Postdoctoral Program for Innovative Talents [BX201700157]
- Shanghai Science and Technology Commission [18JC1411900]
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The metabolic properties of leukemia-initiating cells (LICs) in distinct bone marrow niches and their relationships to cell-fate determinations remain largely unknown. Using a metabolic imaging system with a highly responsive genetically encoded metabolic sensor, SoNar, we reveal that SoNar-high cells are more glycolytic, enriched for higher LIC frequency, and develop leukemia much faster than SoNar-low counterparts in an MLL-AF9-induced murine acute myeloid leukemia model. SoNar-high cells mainly home to and locate in the hypoxic endosteal niche and maintain their activities through efficient symmetric division. SoNar can indicate the dynamics of metabolic changes of LICs in the endosteal niche. SoNar-high human leukemia cells or primary samples have enhanced clonogenic capacities in vitro or leukemogenesis in vivo. PDK2 fine-tunes glycolysis, homing, and symmetric division of LICs. These findings provide a unique angle for the study of metabolisms in stem cells, and may lead to development of novel strategies for cancer treatment.
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