Journal
CELL HOST & MICROBE
Volume 24, Issue 6, Pages 833-+Publisher
CELL PRESS
DOI: 10.1016/j.chom.2018.11.002
Keywords
-
Categories
Funding
- National Research Foundation of Korea [NRF-2017R1A2B3002132]
- Asan Medical Center [2018-678]
- Korean Healthcare Technology R&D Project, Ministry of Health, Welfare and Family Affairs, Republic of Korea [HI13C0016]
- Asan Institute for Life Sciences
Ask authors/readers for more resources
Symbionts play an indispensable role in gut homeostasis, but underlying mechanisms remain elusive. To clarify the role of lactic-acid-producing bacteria (LAB) on intestinal stem-cell (ISC)-mediated epithelial development, we fed mice with LAB-type symbionts such as Bifidobacterium and Lactobacillus spp. Here we show that administration of LAB-type symbionts significantly increased expansion of ISCs, Paneth cells, and goblet cells. Lactate stimulated ISC proliferation through Wnt/beta-catenin signals of Paneth cells and intestinal stromal cells. Moreover, Lactobacillus plantarum strains lacking lactate dehydrogenase activity, which are deficient in lactate production, elicited less ISC proliferation. Pre-treatment with LAB-type symbionts or lactate protected mice in response to gut injury provoked by combined treatments with radiation and a chemotherapy drug. Impaired ISC-mediated epithelial development was found in mice deficient of the lactate G-proteincoupled receptor, Gpr81. Our results demonstrate that LAB-type symbiont-derived lactate plays a pivotal role in promoting ISC-mediated epithelial development in a Gpr81-dependent manner.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available