Journal
CELL BIOLOGY INTERNATIONAL
Volume 43, Issue 1, Pages 22-32Publisher
WILEY
DOI: 10.1002/cbin.11073
Keywords
adhesion receptors; apoptosis; cell cycle phases; cell signaling; intracellular calcium ion mobilization; MG-63 osteoblasts
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Funding
- German Research Foundation (DFG) [NE560/7-2, BE2362/2-2]
- DFG Graduate School welisa [1505/2]
- DFG CRC ELAINE [1270/1]
- KarriereWegeMentoring-Programme of the University of Rostock (European Social Fund for Germany)
- KarriereWegeMentoring-Programme of the University of Rostock (state of Mecklenburg-Vorpommern) [ESF/14-SM-A41-0005/15]
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One of the most popular cell lines in osteogenesis studies is the human osteoblastic line MG-63. For cell biological investigation, it is important that the cells remain stable in their phenotype over several passages in cell culture. MG-63 cells can be used to provide fundamental insights into cell--material interaction. The aim of this study is to present a systematic characterization of the physiological behavior of MG-63 cells in the range of passages 5-30. Significant cell physiology processes during the first 24 h, including cell morphology, availability of adhesion receptors, cell cycle phases, as well as the expression of the signaling proteins Akt, GSK3a/b, IkB-alpha, ERK1/2, p38-MAPK, and intracellular calcium ion mobilization, remained stable over the entire range of passages P5-P30. Due to these stable characteristics in a wide range of cell culture passages, MG-63 cells can be considered as a suitable in vitro model to analyze the biocompatibility and biofunctionality of implant materials.
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