Journal
CARBOHYDRATE POLYMERS
Volume 203, Issue -, Pages 193-202Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.09.029
Keywords
Peach gum polysaccharides; Structural characterization; Molecular weight and chain conformation; Gal-3 binding affinity; Anti-Proliferation activities
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Funding
- National Natural Science Foundation of China [C200501, 31571795, 31501815]
- National Key Research and Development Program of China [2016YFD0400805]
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LP100R, LP10R and LP5R were isolated from peach gum by ultrafiltration. They were identified as AG II arabinogalactans composed of mannose, rhamnose, glucuronic acid, galactose, xylose and arabinose, which had a beta-D-(1 -> 6)-galactan backbone and were branched at O-3 and O-4. LP100R, LP10R and LP5R exist in a spherical conformation with the molecular weight of 8.50 x 10(4) g/mol, 4.77 x 10(4) g/mol and 2.40 x 10(4) g/mol, respectively. The binding affinities of LP fractions to galectin-3 (Gal-3) were 0.77 mu M for LP100R, 2.88 mu M for LP10R and 5.15 mu M for LP5R, respectively. Meanwhile, an anti-proliferative assay revealed that LP100R possessed higher anti-proliferative activity against HepG2 cells (IC50, 4.5 mg/mL) and MCF-7 cells (IC50, 0.43 mg/mL) than did LP10R and LP5R, which were in accordance with their binding affinities to galectin-3. Therefore, LP fractions (especially LP100R) might exert the anti-tumor activity by directly inhibiting the Gal-3 mediated proliferation of cancer cells.
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