Calycosin-7-O-β-D-glucopyranoside stimulates osteoblast differentiation through regulating the BMP/WNT signaling pathways

The isoflavone calycosin-7-O-beta-D-glucopyranoside (CG) is a principal constituent Astragalus membranceus (AR) and has been reported to inhibit osteoclast development in vitro and bone loss in vivo. The aim of this study was to investigate the osteogenic effects of CG and its underlying mechanism in ST2 cells. The resulsts show that exposure of cells to CG in osteogenic mineralization process. Mechanistically, CG treatment increased the expression of bone morphogenetic progtein 2 (BMP-2), p-Smad 1/5/8, beta-catenin and Runx2, all of which are regulateors of the BMP- or wingless-type MMTV pathways, respecively. Taken together, the results indicate that CG promotes the osteoblastic differentiation of ST2 cells through regulating the BN1P/WNT signaling pathways. On this basis, CG may he a useful lead compound for improving the treatment of hone decreasing diseases and enhancing bone regeneration. (c) 2015 Chinese Pharmaceutical Association and Institute of Materia Medic, Chinese Academy of Sciences. Production and hosting by Elsevier B.V.