Journal
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
Volume 18, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12906-018-2395-2
Keywords
Traditional Chinese medicine; Chronic kidney disease; Fibrosis; Jian-Pi-Yi-Shen formula; Mitochondrial quality control network
Categories
Funding
- Shenzhen Science and Technology Plan Project [JSGG20141017103353178, JCYJ20160428182041577, ZDSYS201606081515458, JCYJ20170307154652899]
- Natural Science Foundation of Guangdong Province [2015A030310247, 2015A030310252, 2018A030313305]
- Natural Science Foundation of China [81603437, 81804052]
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BackgroundJian-Pi-Yi-Shen Formula (JPYSF), a Chinese herbal decoction with the efficacies of fortify the spleen and tonify the kidney' and activate blood and resolve stasis', is effective for the treatment of chronic kidney disease in clinic. However, the underlying mechanism remains unclear. The aim of this study was to investigate the therapeutic effects and possible mechanisms of JPYSF on retarding chronic kidney disease progression in 5/6 nephrectomized (5/6 Nx) rats.MethodsPerindopril (4mg/kg/d) and JPYSF (2.72g/kg/d) were administrated by gavage to 5/6 Nx rats daily for 6weeks. The therapeutic effects of JPYSF were evaluated by renal function, pathological injury, and fibrosis. The protein levels associated with mitochondrial quality control network were measured by Western blot and immunofluorescence analysis.Results5/6 Nx rats showed obvious decline in renal function as evidenced by increased serum creatinine, blood urea nitrogen, and urinary protein excretion, and significant injury in kidney structure as evidenced by glomerular hypertrophy, tubular atrophy, and interstitial fibrosis. Administration of JPYSF for 6 weeks could improve renal function and ameliorate kidney structure injury in 5/6 Nx rats. Furthermore, the remnant kidneys of 5/6 Nx rats showed unbalanced mitochondrial quality control network manifested as decreased mitochondrial biogenesis, fusion, and mitophagy, and increased mitochondrial fission. Treatment of JPYSF could restore aforesaid aspects of mitochondrial quality control network.ConclusionsThese results indicate that JPYSF can notably ameliorate 5/6 Nx-induced chronic kidney disease, which may be related with modulation of mitochondrial quality control network.
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