Journal
BIOTECHNIQUES
Volume 65, Issue 4, Pages 191-196Publisher
FUTURE SCI LTD
DOI: 10.2144/btn-2018-0021
Keywords
bone; cartilage; chondrocytes; cryo-sectioning; demineralization; fluorescent imaging; growth plate; histology; mouse; osteoblasts; transgenic
Funding
- NIH NIAMS [AR064462, AR069700]
- USC STAR Program
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The use of fluorescent tags to monitor protein expression and to lineage-trace cells has become a standard complement to standard histological techniques in the fields of embryology, pathology and regenerative medicine. Unfortunately, traditional paraffin embedding protocols can substantially diminish or abolish the native emission signal of the fluorophore of interest. To preserve the fluorescent signal, an alternative is to use cryosectioning; however, this can often result in undesirable artefacts such as tearing or shattering - particularly for mineralized tissues such as bone and cartilage. Here we present a method of using a commercially available tape to stabilize murine femur tissue, thus allowing for cryosectioning of cartilage and bone tissues carrying fluorescent tags without the need for demineralization.
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