Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 83, Issue 4, Pages 755-762Publisher
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2018.1559720
Keywords
13-propanediol; YqhD; 3-Hydroxypropionic acid; co-production; Lactobacillus reuteri
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Funding
- Department of Science and Technology, India [SR/SO/BB-39/2008]
- Department of Biotechnology, Government of India
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The process economy of food grade 1,3-propanediol (1,3-PD) production by GRAS organisms like Lactobacillus reuteri (L. reuteri), is negatively impacted by the low yield and use of expensive feedstocks. In order to improve the process economy, we have developed a multiproduct process involving the production of three commercially important chemicals, namely, 1,3-PD, lactate and 3-Hydroxypropionic acid (3-HP), by engineered L. reuteri. The maximum 1,3-PD and lactate titer of 41 g/L and 31 g/L, with a volumetric productivity of 1.69 g/L/h and 0.67 g/L/h were achieved, respectively. The maximum 3-HP titer of 5.2 g/L with a volumetric productivity of 1.3 g/L/h, was obtained by biotransformation using cells recovered from the repeated fed-batch process. The volumetric productivity of 1,3-PD obtained in this study is the highest ever reported for this organism. Further cost reduction can be achieved by using waste feedstocks like milk whey, biomass hydrolysate, and crude glycerol.
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