Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 83, Issue 1, Pages 181-184Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2018.1527210
Keywords
Ergothioneine; molecular breeding; heterologous expression; Aspergillus oryzae; Neurospora crassa
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Funding
- MEXT, Japan (JSPS KAKENHI) [16H06452]
- JSPS [15H03110, 18H03937]
- Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry from the Ministry of Agriculture, Forestry and Fisheries (MAFF) [26027AB]
- Grants-in-Aid for Scientific Research [15H03110] Funding Source: KAKEN
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To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type).
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